CD4+ T cell Regulation of Immune Responses to the Simian Virus 40 Large Tumor Antigen is Complex and Involves Multiple Layers of Coordination Dependent on Immune Conditions
Date of Award
Bachelor of Science (BS)
Dr. Lawrence Mylin
Cytotoxic (CD8+) and Helper (CD4+) T lymphocytes play an important role in the immune detection and destruction of tumors. The induction of Cytotoxic T cell responses by multiple CD8 epitopes located within the Simian Virus 40 Large Tumor Antigen (SV40 T ag) oncoprotein has been well characterized, and we have begun to characterize the role(s) of CD4+ Helper T cells in controlling cellular immune responses to the SV40 T ag. The goal of this study was to characterize the cytokines produced by activated SV40T ag-specific Helper T cells in order to identify subset(s) of Helper T cells that may differentially influence regulation of the cellular immune response. Mice were immunized with B6/K-145 cells, which express a SV40 T ag mutant in which the CD8 epitopes I, II/III, IV and V have been inactivated.Frequencies of CD4 epitope 381-, 529-and 581-specific T cells expressing the cytokines IFN-, IL-2, or IL-10 were compared using single and double color ELISPOT assays following 24 of 40 hours of in vitropeptide re-stimulation. The data suggests that distinct populations of Helper T cells may secrete IFN-, IL-2 or both cytokines. Additionally, the ratio of IL-10 to IFN-secreting Helper T cells was reduced in the absence accompanying robust Cytotoxic T cell immune response. Our results begin to support a model in which multiple subsets of Helper T cells, potentially TH1 and Treg, work in parallel to coordinate immune responses to cellular tumor antigens.
Raugh, Arielle and Mylin, Lawrence, "CD4+ T cell Regulation of Immune Responses to the Simian Virus 40 Large Tumor Antigen is Complex and Involves Multiple Layers of Coordination Dependent on Immune Conditions" (2016). Honors Projects and Presentations: Undergraduate. 32.