The Search for Plasmodium Falciparum Histidine-Rich Protein 2/3 Deletions in Zambia and Implications for Plasmodium Falciparum Histidine-Rich Protein 2-Based Rapid Diagnostic Tests
Weattempted to identify Plasmodium falciparum histidine-rich protein 2/3 (pfhrp2/3) deletions among rapid diagnostic test (RDT)-negative but PCR- or microscopy-positive P. falciparum-infected individuals in areas of low transmission (Choma District, 2009-2011) and high transmission (Nchelenge District, 2015-2017) in Zambia. Through community-based surveys, 5,167 participants were screened at 1,147 households by P. falciparum histidine-rich protein 2 (PfHRP2)-based RDTs. Slides were made and dried blood spots were obtained for molecular analysis. Of 28 samples with detectable P. falciparum DNA, none from Nchelenge District were pfhrp2/3 negative. All eight samples from Choma District had detectable pfhrp3 genes, but pfhrp2 was undetectable in three. DNA concentrations of pfhrp2-negative samples were low (< 0.001 ng/μL). These findings suggest that PfHRP2-based RDTs remain effective tools for malaria diagnosis in Nchelenge District, but further study is warranted to understand the potential for pfhrp2/3 deletions in southern Zambia where malaria transmission declined over the past decade.
Kobayashi, T.; Sikalima, J.; Parr, J. B.; Chaponda, M.; Stevenson, J. C.; Thuma, Philip; Mulenga, M.; Meshnick, S. R.; and Moss, W. J., "The Search for Plasmodium Falciparum Histidine-Rich Protein 2/3 Deletions in Zambia and Implications for Plasmodium Falciparum Histidine-Rich Protein 2-Based Rapid Diagnostic Tests" (2019). Biology Educator Scholarship. 147.
Kobayashi, T., et al. (2019). The search for plasmodium falciparum histidine-rich protein 2/3 deletions in Zambia and implications for plasmodium falciparum histidine-rich protein 2-based rapid diagnostic tests. American Journal of Tropical Medicine and Hygiene 100(4) 842-845.
© 2019 American Journal of Tropical Medicine and Hygiene. Original published version available at https://doi.org/10.4269/ajtmh.18-0859.
Kobayashi, T., Sikalima, J., Parr, J. B., Chaponda, M., Stevenson, J. C., Thuma, P. E., Mulenga, M., Meshnick, S. R., Moss, W. J., & for the Southern and Central Africa International Centers of Excellence for Malaria Research. (2019). The Search for Plasmodium falciparum histidine–rich protein 2/3 Deletions in Zambia and Implications for Plasmodium falciparum histidine-rich protein 2-Based Rapid Diagnostic Tests. The American Journal of Tropical Medicine and Hygiene, 100(4), 842–845. https://doi.org/10.4269/ajtmh.18-0859