Prevalence of a Variant Gastrin Receptor RNA and Correlating Genomic Polymorphism in Human Pancreatic Cancer
Date of Award
Bachelor of Science (BS)
Currently, the five-year survival rate of pancreatic cancer is an abysmal 5%. Our lab has focused on the etiology of CCK2i4svR, a hyperactive splice variant of the gastrin receptor (CCK2R), which has been associated with increased pancreatic tumor aggressiveness. To determine if a correlation exists between a single nucleotide polymorphism (SNP) in the receptor, and expression of the variant RNA in patients, we aim to genotype human pancreatic tumor samples and quantify CCK2R and CCK2i4svR mRNA. Initial analysis of RNA samples by Real Time RT‐PCR has demonstrated successful quantification of CCK2R expression in both tumor and normal tissue samples as well as very low levels of CCK2i4svR transcript. A higher frequency of splice variant receptor was detected in normal pancreatic tissue. Ongoing efforts will bolster sample size and test for the misspliced receptor in true normal samples.
Jones, Rebekah, "Prevalence of a Variant Gastrin Receptor RNA and Correlating Genomic Polymorphism in Human Pancreatic Cancer" (2019). Honors Projects and Presentations: Undergraduate. 192.