Date of Award
Dr. Lawrence Mylin
The results of this study are part of our ongoing effort to understand factors which control the efficiency with which tumor epitope-specific CD8+ T lymphocytes can control the progression of SV40 Tag-induced tumors in murine models. We wish to explore the requirement and role of SV40 Tag-specific CD4+ T cells in establishing and maintaining tumor control and regulating tumor-induced CD8+ T cell tolerance. We have recently identified residues 529-543 of SV40 Tag as a CD4+ class II epitope. Splenocytes from mice immunized with B6/K-1,4,5 cells were used to search for additional epitopes within SV40 Tag. Peptide immunizations were also used to compare the immunogenicity of two epitopes that induce CD4+ T cells responses: LT529-543 from SV40 Tag and LT678-690 from murine Polyomavirus Large T antigen (mPyT). While the mPyT LT678-690 peptide was shown to be strongly immunogenic within the context of mPyT viral infections by others (Lin et al., 2010), synthetic mPyT LT678-690 and SV40 Tag LT529-543 peptides induced similar, but low levels of active CD4+ T cells. Cells immunized with each peptide in combination with the HBV helper peptide showed a weak response to the SV40 Tag peptide when compared to the mPyT peptide. These results suggest that the lack of virus-induced immunity may limit the immunological potency of the mPyT 678-690 epitope, but that in the presence of a potent helper peptide, the mPyT peptide is about twice as potent an immunogen as the SV40 epitope.
Miller, Elizabeth A.; Hallowell, Benjamin D.; and Mylin, Lawrence M., "Comparing the Immunological Potencies of Two Viral Epitopes: LT529-543 from the Simian Virus 40 Large Tumor Antigen vs. LT678-690 from the Large Tumor Antigen of Murine Polyomavirus" (2012). Honors Projects and Presentations: Undergraduate. 143.