Generation of a T Cell Receptor Transgenic Mouse; Assembly of the Full-sized Expression Cassette
Date of Award
2012
Document Type
Thesis
Department
Biological Sciences
First Advisor
Dr. Lawrence Mylin
Abstract
The development of a T cell receptor transgenic mouse would be useful in research concerning the immune response to tumor cells. By producing a line of mice expressing T cells specific for a particular antigen, Simian virus 40 large tumor antigen, would allow us to harvest large numbers of clonal T cells. These T cells would prove useful for future adoptive transfer experiments to consider the role of CD4+ and CD8+ T cells in the immune response to tumor cells. T cell DNA information encoding a CD4+ T cell receptor specific for the SV40 Tag epitope 529-543 was used to construct an expression cassette vector for use in microinjection of a mouse embryo and the production of a C57B1/6 T cell receptor transgenic mouse. The transgenic mouse line would be very useful for the extraction of T cells for use in adoptive transfer experiment to improve our knowledge of the mammalian immune system specifically its role in tumor prevention and control.
Recommended Citation
Kreider, Daniel L., "Generation of a T Cell Receptor Transgenic Mouse; Assembly of the Full-sized Expression Cassette" (2012). Honors Projects and Presentations: Undergraduate. 141.
https://mosaic.messiah.edu/honors/141
