Title

Generation of a T Cell Receptor Transgenic Mouse; Assembly of the Full-sized Expression Cassette

Date of Award

2012

Document Type

Thesis

First Advisor

Dr. Lawrence Mylin

Abstract

The development of a T cell receptor transgenic mouse would be useful in research concerning the immune response to tumor cells. By producing a line of mice expressing T cells specific for a particular antigen, Simian virus 40 large tumor antigen, would allow us to harvest large numbers of clonal T cells. These T cells would prove useful for future adoptive transfer experiments to consider the role of CD4+ and CD8+ T cells in the immune response to tumor cells. T cell DNA information encoding a CD4+ T cell receptor specific for the SV40 Tag epitope 529-543 was used to construct an expression cassette vector for use in microinjection of a mouse embryo and the production of a C57B1/6 T cell receptor transgenic mouse. The transgenic mouse line would be very useful for the extraction of T cells for use in adoptive transfer experiment to improve our knowledge of the mammalian immune system specifically its role in tumor prevention and control.

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